PMC

(A) Rosette diameter of wild-type and cle mutant plants at the floral transition. Values shown are mean ± standard deviation (S.D.). Asterisks indicate a significant difference from wild-type at p <0.001 (two-tailed Student’s t test). n = 14–20 individuals per genotype. (B) Wild-type Col-0 rosette at the floral transition. (C) cle16-2 rosette. (D) cle16-3 rosette. (E) cle17-2 rosette. (F) cle17-3 rosette. (G) cle27-cr1 rosette. (H) cle27-2 rosette. Scale bar, 1 cm.

Mechanisms of rosette formation. (A) Rosette formation via apical constriction. Prior to apical constriction, cytoskeletal molecules, including F-actin and myosin-II, Par-3 and N-cadherin, are in the apical domains of columnar cells. Apical constriction leads to the formation of a rosette around an acto-myosin-rich center. Note that only apical, but not basal, domains of rosette cells are constricted. Following rosette formation, rosette centers may open to form a central lumen. (B) During rosette formation via planar polarized constriction, cytoskeletal molecules are distributed in a planar polarized fashion throughout the developing tissue, prior to rosette formation. Actin and myosin-II are localized to the AP axis, and Par-3 (Baz) and cadherin are localized to perpendicular membranes. This distribution allows the formation of a rosette with a myosin-II-rich center. Note that cells constrict along their lateral edges. Rosettes formed in this manner often resolve along the axis perpendicular to that of the initial cellular arrangement. Dashed lines indicate the plane of the cross-sections shown.

Quantitative Analysis of gpa1 and agb1 Developmental Phenotypes from Plants Grown under Standardized Nonstressed Conditions.
Data representing 40 traits from the mutant lines are presented in units of standard error from the wild-type mean. Values >2 or <−2 are considered significant (P < 0.05), and the corresponding thresholds are indicated with gray lines.
(A) Scatterplot of data from gpa1-1 versus gpa1-2.
(B) Scatterplot of data from agb1-1 versus agb1-2.
(C) Scatterplot of data from gpa1-2 versus agb1-2.
Trait descriptions (see , for details) are as follows: # seeds/half, number of fully developed seeds on one side of a mature silique; # bolts >1cm, number of inflorescence bolts > 1 cm in length per plant at the completion of flowering (growth stage 6.9); # open flw, number of open flowers per plant when >50% of the experimental population had completed flowering (growth stage 6.9); # rosette lvs, number of rosette leaves produced before the transition to flowering (growth stage 5.10); # senescent flws, number of senescent flowers per plant when >50% of the experimental population had completed flowering (growth stage 6.9); # siliques, number of siliques produced per plant; # split siliques, number of split siliques per plant at the completion of flowering (growth stage 6.9); # stem branches, number of lateral branches emanating from the primary inflorescence at the completion of flowering (growth stage 6.9); flw present?, days to first bud observed (growth stage 5.1); flw width, maximum width across the face of the open flower (average of three flowers per plant); flw cessation, days to the cessation of flowering (growth stage 6.9); flw open, days to first flower opening (growth stage 6.0); lateral roots, number of lateral roots per seedling; max rosette rad., maximum rosette radius achieved during the life of the plant; pedicel length; length of pedicel of the mature second flower (growth stage 6.5); root length, length of the primary root (growth day is given in parentheses); rosette area, exposed leaf area of rosette when the first flower is open (growth stage 6.0); rosette DW, dry weight of rosette (growth stage given in parentheses); rosette eccentricity, rosette shape quantitation (growth stage 6.0); rosette major and minor axes, rosette size quantitation (growth stage 6.0); rosette perimeter, perimeter of intact rosette (growth stage 6.0); rosette S.D. radius, rosette shape quantitation (growth stage 6.0); seed area, area of individual seeds; seed eccentricity, shape quantitation of individual seeds; seed major and minor axes, size quantitation of individual seeds; seed perimeter, perimeter of individual seeds; seed S.D. radius, shape quantitation of individual seeds; seed wt./plant DW, dry weight of seed produced per plant; seed wt./plant FW, fresh weight of seed produced per plant; seedling FW, fresh weight of the entire seedling at day 14; sepal length, sepal length (average of three flowers per plant); silique length, silique length (average of three siliques per plant); silique DW, dry weight of siliques per plant (growth stage 6.9); stem DW, dry weight of inflorescence stem tissue per plant.

Example 1: determination of projected rosette area with Rosette Tracker. A, Original color image showing Columbia-0 plants. B, Segmentation image produced from the color image by Rosette Tracker. Centered numbers in the rosettes from the Rosette Tracker image were repeated on the picture, next to the plant, for clarity. C, Graph showing the projected rosette area per plant from A, as calculated by Rosette Tracker. [See online article for color version of this figure.]